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Opiates assay

Product Method Size Catalog Price Quantity
Opiates assay Homogenous EIA R1 2 x 16.9ml, R2 2 x 8ml DA4012 $647.20
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  • Format
    Liquid ready to use
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    28 days
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Intended Use

The Opiates assay is an in vitro diagnostic reagent. For the qualitative and semi-quantitative analysis of Opiates in human urine. The cut-off for the qualitative application is 2000ng/ml in accordance with SAMHSA recommendations. This product is suitable for use on the RX Series instruments, which includes the Rx Daytona and the Rx Imola.

This assay provides only a preliminary result. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly in evaluating a preliminary positive result. To obtain a confirmed analytical result, a more specific alternate chemical method is needed. Gas chromatography/mass spectroscopy (GC/MS) is the recommended confirmatory method.


Clinical Significance

Opiates are naturally occurring alkaloids derived from the opium poppy, Papaver somniferum. Common opiates include morphine, codeine, and heroin, which is a semi-synthetic derivative of morphine.

Morphine and Codeine are potent analgesics. They are among the most effective and common medications for treatment of mild to severe pain. These legitimate drugs, however are frequently abused for their central nervous system (CNS) effects. Heroin is the most commonly abused opiate. It may be snorted, smoked, or dissolved and injected subcutaneously or intravenously.

Opiates are absorbed rapidly, and primarily metabolized in liver. Heroin is converted quickly to 6-acetylmorphine or morphine, which is excreted in urine both unchanged and as glucuronide conjugates. Excretion takes place over 2 to 3 days. Codeine is excreted in urine as glucuronides, or norcodeine, or as morphine. The presence of opiates in urine indicates the use of heroin, morphine, codeine, and/or other synthetic opiates structurally related to morphine, such as oxycodone.


Principle

The assay is based on competition between drug in the sample and drug labeled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH) for a fixed amount of antibody in the reagent. Enzyme activity decreases upon binding to the antibody, and the drug concentration in the sample is measured in terms of enzyme activity.

In the absence of drug in the sample, morphine-labeled G6PDH conjugate is bound to antibody, and the enzyme activity is inhibited. However, when free drug is present in the sample, antibody would bind to free drug; the unbound morphine-labeled G6PDH then exhibits its maximal enzyme activity.

Active enzyme converts nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that can be measured spectrophotometrically at 340 nm.


Available Applications

  • Various