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Opiates assay

Product Method Size Catalog Price Quantity
Opiates assay Homogeneous Enzyme Immunoassay R1 2 x 16.9ml, R2 2 x 8ml DA4012 $647.20
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  • Format
    Liquid ready-to-use
  • Assay Range
    -
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

The Opiates assay is an in vitro diagnostic test for the qualitative and semi-quantitative detection of Opiates in human urine on the RX imola and RX daytona analysers at the 2000 ng/ml cut-off. The assay is calibrated against morphine.

This assay provides only a preliminary result. A more specific alternative chemical method must be used in order to obtain a confirmed analytical result. Gas Chromatography/ Mass Spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgement should be exercised with any drug of abuse test result, particularly when a preliminary result is positive.

Clinical Significance

Opiates are naturally occurring alkaloids derived from the opium poppy, Papaver somniferum. Common opiates include morphine, codeine, and heroin, which is a semi-synthetic derivative of morphine.

Morphine and Codeine are potent analgesics. They are among the most effective and common medications for treatment of mild to severe pain. Heroin is the most commonly abused opiate. It may be snorted, smoked, or dissolved and injected subcutaneously or intravenously.

Opiates are absorbed rapidly, and primarily metabolised in liver. Heroin is converted quickly to 6-acetylmorphine or morphine, which is excreted in urine both unchanged and as glucuronide conjugates. Excretion takes place over 2 to 3 days. Codeine is excreted in urine as glucuronides, or norcodeine, or as morphine.

Principle

The assay is based on competition between drug in the sample and drug labelled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH) for a fixed amount of antibody in the reagent. Enzyme activity decreases upon binding to the antibody, and the drug concentration in the sample is measured in terms of enzyme activity.

In the absence of drug in the sample, morphine-labelled G6PDH conjugate is bound to antibody, and the enzyme activity is inhibited. However, when free drug is present in the sample, antibody would bind to free drug; the unbound morphine-labelled G6PDH then exhibits its maximal enzyme activity.

Active enzyme converts nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that can be measured spectrophotometrically at 340 nm.

Available Applications

RX series instruments, including:

RX daytona

RX imola