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Phenobarbital assay

Product Method Size Catalog Price Quantity
Phenobarbital assay LEI R1 2 x 17ml, R2 2 x 6ml TD3408 $640.33
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  • Format
    Liquid ready to use
  • Assay Range
    2.55 - 87.7ug/ml
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of Phenobarbital in human serum. This product is suitable for use on the RX Series instruments which includes the Rx Daytona and Rx Imola.


Clinical Significance

It is essential to maintain a constant concentration of medication in order for treatment to be successful, insufficient levels can mean drugs are not effective and excessive levels in some cases can lead to toxicity.

Often there is little difference between the dosage required for effective treatment and the dosage needed to cause severe side effects and even toxicity. The fact that individuals absorb, metabolize and make use of drugs at different rates means a standard dose cannot be prescribed for everyone. It is therefore extremely important to monitor the use of drugs with a narrow therapeutic range to ensure individuals are receiving the optimum treatment.

Phenobarbital is an antiepileptic and sedative-hypnotic drug. Measurements are used in the diagnosis and treatment of phenobarbital use or overdose and in monitoring levels of phenobarbital to ensure appropriate therapy.


Principle

Randox Phenobarbital assay is a latex-enhanced immunoturbidimetric assay. It is based on the principle of measuring changes in scattered light. The latex particles are coated with phenobarbital, which in the presence of phenobarbital antibody solution, rapidly agglutinate.

When a sample containing phenobarbital is introduced, the agglutination reaction is partially inhibited, slowing down the agglutination process. The rate of agglutination is inversely dependent upon the concentration of phenobarbital in the sample. By monitoring the change in scattered light, as a change in absorbance, a concentration curve can be obtained. The actual change in absorbance is inversely proportional to the concentration of phenobarbital in the sample.

This assay uses an end point method and multipoint calibration.


Available Applications

  • Various