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Phosphorus (inorganic) assay

Product Method Size Catalog Price Quantity
Phosphorus (inorganic) assay UV 480 tests PH2810 $206.91
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  • Format
    Mixing and diluting are automatically performed by the Dimension® System.
  • Assay Range
    0.112 - 6.65mmol/l
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative determination of phosphorus in serum. This product is suitable for use on the Siemens Dimension® analyzers.


Clinical Significance


The human body contains approximately one kilogram of phosphorous. The calcium phosphate salts which comprise the inorganic substance of bone account for approximately 80% of the total phosphorous content. The remainder is distributed throughout other cells of the body primarily as organic phosphorous in phospholipids and phosphoproteins. In serum most inorganic phosphorus exists in a free form with approximately 15% bound to protein.

Abnormal levels of serum phosphates are most commonly seen in kidney, bone and parathyroid diseases. Phosphate is usually measured in conjunction with serum calcium since each measurement is useful in the interpretation of the other.

Lower than normal levels of phosphorous may be the result of malnutrition, ketoacidosis, use of diuretics, hyperparathyroidism or hypothyroidism. Elevated levels are often the result of kidney failure or hypoparathyroidism.


Principle


The phosphorous (PHOS) method is a modification of the classical phosphomolybdate method introduced by Fiske and Subbarow and uses a mixture of p-methylaminophenol sulfate and bisulfite to reduce the phosphomolydbate as reported by Gomori and by Drewes. The PHOS method measures the absorption of the reduced phosphomolybdate complex at UV wavelengths to improve sensitivity. Because of the pre-blanking of the sample, bilirubin interference is considerably minimized.

Inorganic phosphorous reacts with ammonium molybdate in the presence of sulphuric acid to form a phosphomolybdate complex which is measured at 340 nm.


Available Applications

  • Dimension dedicated