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Sldl-c assay

Product Method Size Catalog Price Quantity
Sldl-c assay CLEARANCE R1 1x18, R2 1x6ml 562616 $1607.78
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Intended Use

Enzymatic Clearance assay for the in vitro quantitative determination of small, dense LDL-cholesterol (sLDL-C) in human serum and plasma.


Clinical Significance


Small LDL cholesterol (sLDL) is a subtype of LDL cholesterol, one of the lipoproteins. LDLs vary in size through genetic determination and dietary lipid intake, ranging from small and dense to large and buoyant LDL. They all transport triglycerides and cholesterol to the tissues, but their atherogenesis varies according to size. Smaller particles such as sLDL more readily permeate the inner arterial wall and are more susceptible to oxidation.

LDL cholesterol is a major risk factor for cardiovascular disease (CVD). sLDL is particularly atherogenic; a person with elevated sLDL levels has a 3-fold increased risk of myocardial infarction (MI). Elevated levels of LDL arise from multiple sources. A major factor is sedentary lifestyle with a diet high in saturated fat. Insulin-resistance and pre-diabetes have also been implicated, as has genetic predisposition.

Measurement of sLDL allows the clinician to get a more comprehensive picture of lipid risk factors and tailor treatment accordingly. Reducing LDL levels will reduce the risk of CVD and MI. Regular check-ups are needed to assess the balance between LDL and HDL cholesterol and, if commenced, monitor treatment.

To date, ultracentrifugation and electrophoresis-based methods are used for the measurement of sdLDL cholesterol however these methods are both laborious and time-consuming. The sLDL-EX “SEIKEN” test is a direct method for the quantitative determination of sdLDL cholesterol, using automated chemistry analyzers capable of accommodating two-reagent assays. The test is completed within 10 minutes.


Principle

First step: sLDL is separated from the other LDL subclasses by the action of polyanion and divalent cations in the sample pre-treatment solution.

Second step: on clinical chemistry analyzer (fully automated) Reagent 1 decomposes HDL through a HDL-specific surfactant, then cholesterol is degraded enzymatically to water and oxygen.