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Total Iron Binding Capacity 2 (TIBC-2) assay

Product Method Size Catalog Price Quantity
Total Iron Binding Capacity 2 (TIBC-2) assay Colorimetric R1 4 x 8.7ml, R2 4 x 4.9ml TI8065 $736.56
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  • Format
    Liquid ready-to-use
  • Assay Range
    37.8 - 957μg/dl
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

A TIBC test system is a device intended for the quantitative in vitro determination of total iron-binding capacity (TIBC) concentration in serum. This product is suitable for use on the RX series analyser RX modena.

Clinical Significance

Measurements obtained by this device are used in the diagnosis and treatment of anemia.

Principle

Randox TIBC assay uses two reagents in a sequential process that may be monitored spectrophotometrically.

Step 1:

The first reagent (R1) an acidic buffer containing an iron-binding dye and ferric chloride is added to the serum sample. The low pH of R1 releases iron from transferrin. The iron then forms a coloured complex with the dye. The coloured complex at the end of this first step represents both the serum iron and excess iron already present in R1.

Step 2:

Reagent 2 (R2), a neutral buffer is then added, shifting the pH and resulting in a large increase in affinity of transferrin for iron. The serum transferrin rapidly binds the iron by abstracting it from the dye-iron complex. The observed decrease in absorbance of the coloured dye-iron complex is directly proportional to the total iron-binding capacity of the serum sample.

Available Applications

RX Modena