Iron (serum only) assay
| Product | Method | Size | Catalog | Price | Quantity |
| Iron (serum only) assay | Colorimetric | 2 x 100ml | SI257 | £81.25 | |
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- Format
Lyophilised - Assay Range
0.964 - 152µmol/l - Working Stability 15-25 °C
_ - Working Stability 2-8 °C
4 weeks - Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of Iron in serum. This product is suitable for both manual and automated use.
Clinical Significance
The concentration of serum iron reflects principally the concentration of ferric iron bound to serum transferrin, a protein responsible for transporting iron from the intestine to the cells were it is needed.
Iron deficiency is one of the most prevalent human disorders. In adults iron deficiency is often symptomatic of chronic blood loss. Iron measurements are particularly beneficial when determining if anaemia is due to iron deficiency or long term illness. Serum iron concentrations also appear to be decreased in acute or chronic inflammatory disorders such as acute infection, immunisation or myocardial infarction.
Elevated levels of serum iron are present in haemochromatosis a genetic condition where too much iron is present in the body. Higher than normal levels of iron are also known to occur as a result of blood transfusion, hepatic disease, renal disease and lead poisoning.
Principle
Ferric iron is dissociated from its carrier protein, transferrin, in an acid medium and simultaneously reduced to the ferrous form. The ferrous iron is then complexed with the chromogen, a sensitive iron indicator, to produce a blue chromophore which absorbs maximally at 595 nm.
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